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Most considerations of non-animal alternatives for skin irritation/sensitization testing tend to examine a single aspect of the process (e.g., chemical reactivity, epidermal bioavailability, dendritic cell responses)
Give topical GHK-Cu several weeks of consistent use
In this study, we investigated the skin toxicity of several copper compounds using cell-based in vitro tests and evaluated the expression of various cytokines at genomic and proteomic levels to discover irritancy biomarkers suitable for copper compounds
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